问题 学科领域 问题类型
如何根据文章题目检索文献? 医药科学 文献查找
答案
读者(15:32:27): 怎么搜文章? 咨询员(15:32:28): 检索文献是将检索式填写在检索框中,勾选上文献类型 咨询员(15:33:16): 在这里:http://168.160.2.82/NSTL/facade/search/seniorSearch.do 咨询员(15:35:25): 网上订购的步骤: 1.用户注册成为普通用户; 2.交费成为付费用户,收费标准与付费方式在主页右上角“帮助中心”; 3.登录; 4.点击“文献检索”,选择数据库后,检索; 5.显示检索结果并选择; 6.放入购物车,然后依据系统提示操作,直至生成订单。 下订单后24小时之内NSTL的工作人员会把文献发送到用户的信箱里。 读者(15:42:46): 我把文章名让进去。 读者(15:42:54): 选了西文,可是不行。 读者(15:42:56): 搜不到。 咨询员(15:55:26): 您要查什么,我帮您试试。 读者(15:56:01): Large-scale bacterial fermentation and isolation of scFv multimers using a heat-inducible bacterial expression vector 咨询员(15:58:41): 是这篇吗? 咨询员(15:58:44): Large-scale bacterial fermentation and isolation of scFv multimers using a heat-inducible bacterial expression vector. Bayly AM;Kortt AA;Hudson PJ;Power BE; 【作者单位】:CSIRO Health Sciences and Nutrition, 343 Royal Parade, Parkville, Victoria, 3052, Australia【刊名】:Journal of Immunological Methods【ISSN】:0022-1759【出版年】:2002【卷】:262【期】:1/2【起页】:217【止页】:227【总页数】:11【分类号】:R392【关键词】:Proteins;Fermentation;Unmarried;Heat;Antibodies;蛋白质类;发酵;热;抗体;【语种】:英语【文摘】: This protocol describes optimised large-scale bacterial fermentation conditions for recombinant single-chain Fv molecule (scFv) monomers and multimers (diabodies and triabodies). The heat-inducible bacterial secretion vector, pPOW3, utilising the temperature-regulated tandem lambda promoters is particularly suited to the large-scale fermentation of single-chain antibodies, providing low-cost recombinant protein synthesis. The protein expressed by this vector is secreted into the periplasm where it is found as both the soluble and insoluble protein that is associated with the cell membranes. A protein fractionation method for the rapid extraction and affinity purification of the soluble protein fraction and the urea solubilization and refolding of the insoluble protein fraction expressed from single-chain antibody (Ab) fragment gene constructs is described. This method is simple to perform and utilises inexpensive reagents to provide cost-effective protein synthesis.【馆藏单位】:中国医科院医学信息研究所 读者(15:59:22): 是。 咨询员(15:59:33): 我检索的时候没有输入全部题名,而只输入一部分:Large-scale bacterial fermentation and isolation 读者(15:59:41): 对。 咨询员(15:59:43): 这样就找到了这篇文章。 咨询员(16:00:15): 因为题目中有的地方拼写方式与数据库中的不同,这就造成检索不到。 咨询员(16:00:27): 加入购物车 咨询员(16:00:37): 然后按系统提示一步一步操作。